Ex-Vivo Organ Perfusion

Ex-vivo organ perfusion systems are used to better evaluate and minimize ischemia-reperfusion injury of donor organs. In addition to an increasing role in organ transplantation, ex-vivo organ perfusion has also been recognized as a useful tool for ex-vivo therapy. These are highly innovative strategies, in terms of regenerative, immunological, cell-based, pharmacological, as well as anti-tumor and anti-infective therapeutic approaches for the various ex-vivo perfusable organs / extremities in the context of autologous replantation as well as allogeneic and xenogeneic transplantation, which this research group is working to develop and establish.


PD Dr.
Bettina Wiegmann
Clinic for Cardiac, Thoracic, Transplantation and Vascular Surgery


1. Warnecke G, Moradiellos J, Tudorache I, Kühn C, Avsar M, Wiegmann B, Sommer W, Ius F, Kunze C, Gottlieb J, Varela A, Haverich A. Normothermic perfusion of donor lungs for preservation and assessment with the Organ Care System Lung before bilateral transplantation: a pilot study of 12 patients. Lancet. 2012 Nov 24;380(9856):1851-8. doi: 10.1016/S0140-6736(12)61344-0. epub 2012 Oct 10.

2 Wiegmann B, Haverich A. Ex vivo lung perfusion. Transfusion Medicine 2018; 8(04): 220-225. DOI: 10.1055/s-0043-115524.

3 Figueiredo C, Carvalho Oliveira M, Chen-Wacker C, Jansson K, Höffler K, Yuzefovych Y, Pogozhykh O, Jin Z, Kühnel M, Jonigk D, Wiegmann B, Sommer W, Haverich A, Warnecke G, Blasczyk.

4. immunoengineering of the vascular endothelium to silence MHC expression during normothermic ex vivo lung perfusion. Hum Gene Ther. 2018 Nov 20. doi: 10.1089/hum.2018.117.

4. Warnecke G, Van Raemdonck D, Smith MA, Massard G, Kukreja J, Rea F, Loor G, De Robertis F, Nagendran J, Dhital KK, Moradiellos Díez FJ, Knosalla C, Bermudez CA, Tsui S, McCurry K, Wang IW, Deuse T, Lesèche G, Thomas P, Tudorache I, Kühn C, Avsar M, Wiegmann B, Sommer W, Neyrinck A, Schiavon M, Calabrese F, Santelmo N, Olland A, Falcoz PE, Simon AR, Varela A, Madsen JC, Hertz M, Haverich A, Ardehali A. Normothermic ex-vivo preservation with the portable Organ Care System Lung device for bilateral lung transplantation (INSPIRE): a randomised, open-label, non-inferiority, phase 3 study. Lancet Respir Med. 2018 May;6(5):357-367. doi: 10.1016/S2213-2600(18)30136-X. Epub 2018 Apr 9.

5. Kaltenborn A, Krezdorn N, HoffmannS, Gutcke A, Haastert-Talini K, Vogt P, Haverich A, Wiegmann B; Ex-vivo limb perfusion for traumatic amputation in military medicine; Mil Med Res. 2020 Apr 26;7(1):21. doi: 10.1186/s40779-020-00250-y. PMID: 32334640


6. Calabrese F, Schiavon M, Perissinotto E, Lunardi F, Marulli G, Di Gregorio G, Pezzuto F, Vuljan S, Forin E, Wiegmann B, Jonigk D, MD, Warnecke G, Rea F; Organ Care System Lung resulted In Lower Apoptosis and INOS Expression in Donor Lungs; Am J Transplant. 2020 Jul 11. doi: 10.1111/ajt.16187.

7. Hitz AM, Bläsing KA, Wiegmann B, Bellmàs-Sanz R, Chichelnitskiy E, Wandrer F, Horn LM, Neudörfl C, Keil J, Beushausen K, Ius F, Sommer W, Avsar M, Kühn C, Tudorache I, Salman J, Siemeni T, Haverich A, Warnecke G, Falk CS, Kühne JF. Donor NK and T Cells in the Periphery of Lung Transplant Recipients Contain High Frequencies of Killer Cell Immunoglobulin-Like Receptor-Positive Subsets. Front Immunol. 2021 Dec 13;12:778885. doi: 10.3389/fimmu.2021.778885.

8, Ledwoch N, Wiegmann B, Chichelnitskiy E, Wandrer F, Kühne JF, Beushausen K, Keil J, Radomsky L, Sommer W, Knöfel AK, Rojas SV, Ius F, Haverich A, Warnecke G, Falk CS. Identification of distinct secretory patterns and their regulatory networks of ischemia versus reperfusion phases in clinical heart transplantation. Cytokine. 2022 Jan;149:155744. doi: 10.1016/j.cyto.2021.155744. epub 2021 Oct 11. PMID: 34649160.


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Ex-vivo lung perfusion, ex-vivo heart perfusion, ex-vivo limb perfusion, organ care system, XVIVO system, ex -vivo regeneration, ex-vivo immunotherapy, ex-vivo tumor therapy, ex-vivo infection therapy.

PD Dr. Bettina Wiegmann

+49 511 532 1408



European patent application, "Perfusion System", Application number EP22166337.0