Director of Clinic/Institute: Univ.-Prof. Dr. Dr. Nils-Claudius Gellrich, Klinik und Poliklinik für Mund-, Kiefer- und Gesichtschirurgie, Medizinische Hochschule Hannover
Representative at the NIFE
Dr. Andreas Kampmann, MKG-Forschung im NIFE, OE7721, Medizinische Hochschule Hannover
tissue engineering, implant vascularization, biocompatibility, small animal models
fluorescence microscope equipped with a video device for intravital microscopy
Short summary of scientific expertise
To facilitate the clinical usage of tissue engineered (TE) constructs new strategies are needed that accelerate angiogenesis after implantation of these constructs; especially when these constructs are of a clinical relevant size. Our group verifies and evaluates these strategies by means of intravital fluorescence microscopy. Therefore we use different small animal models that allow the observation of the microcirculation and angiogenesis in different physiological situations: skinfold chamber (mice, soft tissue), calvaria bone chamber (mice and rat, desmal bone) and femur chamber (mice and rat, chondral bone). With these models we are able to follow the dynamic development of the microcirculation inside TE-constructs over a period of app. three weeks repetitive in one animal. This method also allows simultaneous studies of the biocompatibility of the implants. To verify the in vivo results we apply different histological und immunohistological techniques with a special emphasis on techniques related to the analysis of hard tissue.